By treating prostate-cancer cells (C4-2B) transfected with STAT-1/-3 reporters (pGAS/ISRE and pSTAT-3Luc, respectively) with the Nluc-27 fusions, we achieved significant STAT-1 activation in cells, particularly in cells treated with Nluc-27.pepL Both Nluc-27.scr1 and irrel1 untargeted forms also induced STAT-1 activation due to the native activity of IL-27. Here, IL27 is linked to Familial prostate cancer.