Intracellular staining revealed that OVA257-264-specific CD8+ T cells from NAFLD livers had higher levels of the cytotoxic molecule GRZb (Figures 3C and S2B), but similar levels of perforin (Figures S2C and S2E) as compared to normal livers, which was not changed in mice bearing OVAhigh tumor. Here, PRF1 is linked to metabolic dysfunction-associated steatotic liver disease.