Furthermore, we showed that SRSF3 regulated serum response factor (SRF) expression by binding to the “CAUC” motif in exon 6 of SRF pre-mRNA and participated in the splicing of SRF. Moreover, we confirmed that SRF had a proangiogenic effect and that SRSF3 promoted the angiogenesis of CRC by splicing SRF. This evidence concerns the gene SRF and colorectal carcinoma.