PSEN1 and Alzheimer disease: To provide a comprehensive analysis of the autophagic activity in both Aβ- and P-tau-based AD models, we examined the autophagosome maturation process in N2a mouse neuroblastoma cells stably expressing the human Swedish mutant APP695 (N2S cells) (Figure S1A), in a 3xTg AD mice (APP Swedish, MAPT P301L, and PSEN1 M146V) model (Figure S1B) and in a 5xFAD model (M146L and L286V mutations in PSEN1, and Swedish (K670N/M671L), Florida (I716V), and London (V717I) mutations in APP) (Figure S1C).