RILP and Alzheimer disease: To understand whether RAB7 activity is impaired in AD cell and mouse models, we applied the GST-tagged RAB7-binding domain (GST-R7BD) of RILP 19, 24 and GTP beads 19, 25, which selectively bind GTP-binding proteins, to monitor the level of the GTP form of RAB7 (GTP-RAB7) in autophagosome fractions (Figure S1F).