Given the important roles of the Wnt pathway in regulating the stemness of cancer 31 and since both GO and KEGG analysis of our RNA-seq results revealed the down-regulation of the Wnt/β-catenin signaling pathway after KDM1A knockdown, we hypothesized that KDM1A regulated the stemness and chemosensitivity of thyroid cancer through this pathway. The gene discussed is KDM1A; the disease is thyroid cancer.