Our analysis of CDR3 distribution with the D50 index showed clonal expansion exists in CD4+ T cells in the TB microenvironment, which is consistent with previous observations (Jasenosky et al., 2015); moreover, we found that CD4+ T cell clonal expansion is mainly composed of low- and middle-low frequency CDR3 clonotypes in TPE and blood. The gene discussed is CD4; the disease is tuberculosis.