In univariate analyses, family history of stroke [ORs (95% CI): 2.502 (0.929, 6.736)], thalamus infarction [ORs (95% CI): 2.762 (0.847, 9.008)], and high NfL level [ORs (95% CI): 3.665 (1.993, 6.742)] were found to be potential risk factors of anxiety. The gene discussed is NEFL; the disease is Stroke.