For example, recent studies by Go et al. (2016) using a high fat diet (HFD) model of non-alcoholic fatty liver disease (NAFLD) and whole-body pyruvate dehydrogenase kinase 2 (PDK2) knock-out (KO) model demonstrated the interplay between fluxes through pyruvate dehydrogenase (PDH), pyruvate carboxylase (PC), and ketogenesis. The gene discussed is PDP1; the disease is metabolic dysfunction-associated steatotic liver disease.