Therefore, to verify that under experimental conditions, the two tests can detect anxiety-like states in rats, we expose the rats to intermittent footshock using parameters that induce physiological stress responses (increased peripheral corticosterone secretion and extrahypothalamic corticotropin-releasing factor neurotransmission) and reinstatement of drug seeking (Shaham et al., 2000; Mantsch et al., 2016). This evidence concerns the gene CRH and Anxiety.