To determine the cell fate of VAR2 after internalization, tumor cells (PC3, COLO205, and MG63) were treated with rVAR2 for up to 24 h at 37 °C and examined by confocal microscopy for rVAR2 co-localization with markers of the early endosome (EEA1) and lysosome (LAMP1). This evidence concerns the gene EEA1 and neoplasm.