FAS and metabolic dysfunction-associated steatotic liver disease: Interestingly, expression of the Kindlin-2 and Fas mRNA and that of Kindlin-2 protein were all significantly upregulated in livers of patients with NAFLD compared to those in liver samples from nonsteatotic donors (ND), as revealed by western blotting, quantitative real-time polymerase chain reaction (qRT-PCR) analysis and IHC staining (Fig. 1k–n).