In order to address whether the effect of Gnmt deletion was based on prostate cancer cell-autonomous effects, we silenced GNMT in three independent PCa cell lines with differing AR status and GNMT levels (PC3, DU145, and LNCaP) using two different short hairpin RNAs (Fig. 4A–H; Supplementary Fig. S9A–D). The gene discussed is AR; the disease is Familial prostate cancer.