In addition, in the ELISPOT experiment of cellular immunity, the number of spots of activated IFN-γ-secreting effector T cells in the vaccine group was much greater than that of the AD-NC empty group and the PBS blank control group, and the differences were statistically significant (P < 0.05), which showed that mice can effectively produce specific T lymphocytes after vaccination to kill HPV16/18/58 tumor cells and protect mice from HPV16/18/58 tumor cells attacked. This evidence concerns the gene IFNG and neoplasm.