Transient transfection of si-UBE2D2 + pcDNA-NC and si-UBE2D2 + pcDNA-EIF4G2 was into A549 cells to figure out the function of circ-UBE2D2/miR-376a-3p/EIF4G2 axis in the progression of NSCLC, and the successful transfection was via PCR and WB (Figure 6(a)). The gene discussed is UBE2D2; the disease is non-small cell lung carcinoma.