This subjecthas been reviewed.362 In one example, positionalscanning of sequences of a series of decapeptides was used to identifytumor-reactive CD8+ T cell clones specific for the melanomacell antigen Melan-A.363 The same grouplater used this procedure to screen the cytotoxicity of a librarycomposed of 3.1 × 1011 9-mer peptides in a positionalscanning format, to search for antigens recognized by a melanoma-reactiveCTL. Here, MLANA is linked to melanoma.