The Zic4 enhancer drives Gal4 expression in the proliferating regions of the developing CNS and upon binding of Gal4 to its target UAS sequence, activates expression of mCherry in control larvae (hereafter HRasV12−) and additional eGFP‐HRasV12 expression in tumor developing fish (hereafter HRasV12+ larvae). Here, ZIC4 is linked to neoplasm.