Interestingly, many pathways and cell types that were enriched in PM when compared to primary tumours are also features of CMS4 CRC (e.g.: high TGFβ, angiogenesis, complement, stromal fibroblasts, monocytes, macrophages; low WNT and MYC target genes) [3, 25]. This evidence concerns the gene TGFB1 and neoplasm.