TRPC6 and acute kidney injury: To circumvent possible bias due to the genetical approach and to examine effects of pharmacological blockade of Trpc6 on renal damage, we used SH045 (a recently developed drug with high affinity and strong subtype selectivity toward TRPC6) in vivo in the ischemic AKI mouse model using two different ischemia time (milder with 17.5 min and a stronger with 20 min) (Fig. 5A).