A high plasma AST activity is a feature of obesity-induced hepatic steatosis39,40, and we have also shown a potential protective effect of eTRF against high liver enzyme activity, which is consistent with the results of most previous studies of animal models of liver steatosis, non-alcoholic fatty liver disease4,6,41–47, or hepatic ischemia-reperfusion5; only one previous study showed that TRF does not affect the activities of the liver enzymes ALT, ALP, and GGT48. This evidence concerns the gene GPT and Obesity.