TDRKH and hepatocellular carcinoma: After knocking down the expression of TDRKH-AS1 using short hairpin RNAs (shRNA) (p < 0.01 in Huh7, p < 0.001 in MHCC97H, Fig. 8A), we conducted CCK8 and colony formation assays to detect the influence of TDRKH-AS1 on cell growth of HCC in vitro.