Tumor size (P < 0.001), tumor thrombus (P = 0.005), preoperative AFP (P < 0.001) and BCLC stage (P = 0.014 and < 0.001 for B stage and C stage, respectively) were statistically associated with OS in the CHCC cohort (Additional file 10: Table S1). The gene discussed is AFP; the disease is neoplasm.