The overexpression of CDK4 had been verified in the RT-qPCR experiment, but not CDK1. Although the clinical significance of CDK1 was not validated in RT-qPCR experiments, CDK1 is considered a prognostic factor for HCC in various cohorts and online databases, and its clinical significance in HCC needs to be further explored. The gene discussed is CDK4; the disease is hepatocellular carcinoma.