To gain an insight into the molecular mechanisms of KCNQ1OT1 on colon cancer, KCNQ1OT1 siRNA was transfected into HCT116 and SW480 cells, and the successful transfection of KCNQ1OT1 siRNA was validated by the remarkably suppressed expression of KCNQ1OT1 (Figure 4A). Here, KCNQ1OT1 is linked to malignant colon neoplasm.