ACSL1 and infection: 30 Consistently, various defense-related genes, including GCC box-containing PR genes were constitutively expressed in 35S::CaERFLP1 tobacco plants.30 Identified ERFs regulated the expression of ET/JA-responsive genes by targeting GCC box present in their promoters.31 Similarly, CaERF1A also might bind to the GCC box of ACS1 and LOX1 promoters to regulate ET/JA synthesis against TMV and necrotic fungal pathogen infections.