In order to further explore the role of neutrophils in DC mediated Th1 cell polarization in vivo, we also measured mRNA levels of T-bet, a lineage marker of Th1 cell differentiation [24], in CD4+ T cells from ear dLNs of GL113 or 1A8 treated LdWT and LdCen-/- infected mice 5d post infection (S6A Fig). Here, CD4 is linked to infection.