Although a developmental link between those cells and CD4 exhausted cell types was not observed, CD4+ T cells (CD4 T_cyto1) within the positively prognostic T_Tcyto1 cluster expressed relatively higher expression of effector molecules and coinhibitory molecules such as PD-1, indicating that they may be important in immunotherapy, as has been shown for cytotoxic GZMK+CD4+ T cells with a similar gene signature in bladder cancer (64). Here, CD4 is linked to urinary bladder carcinoma.