Then, GS inhibitor l‐Mmethionine sulfoximine (MSO) abolished the increased percentage of OCT4+ cells induced by glutamine deprivation in Huh7 and HepG2 cells, and even completely prevented sphere formation following glutamine withdrawal in liver cancer cell lines and primary liver cancer cells (Figure 2C–E and Figure S4A,B, Supporting Information). Here, POU5F1 is linked to liver cancer.