LMLN and infection: Finally, we tested whether the observed similarly increased levels of footpad swelling on animals co-inoculated with LeishEXO GP63WT and GP63High was occasioned by the use of L. amazonensis GP63WT, as baseline infection, which, considering the already present levels of GP63 on these parasites, would lead the group LeishEXO GP63High to reach of a theoretical biological limit for the action of this virulence factor on the increased cutaneous pathology, we infected male Balb/c mice on their footpads with L. amazonensis GP63Low only or co-inoculated with LeishEXO GP63WT, GP63High, or GP63Low.