Pro-inflammatory genes (TNF-α, IFN-β and IL-1β) are the downstream signals of TLR-4, and the expression of TLR4, TNF-α (Th1 cytokine) and IFN-β in the colon of CRS group were significantly up-regulated while the expression of IL-1β (Th1 cytokine) in the colon tends to be increased in the CRS group compared with the CON group. The gene discussed is TNF; the disease is congenital rubella syndrome.