ZEB1 and pachyonychia congenita: To further investigate whether ZEB1 could affect PC cell invasion and migration via modulation of the EMT process, we detected the effect of ZEB1 on PC cell invasion and migration by Transwell assay, which exhibited decreased invasive and migrative abilities of PANC-1 cells transfected with si-ZEB1, and increased invasive and migrative abilities of AsPC-1 cells transfected with oe-ZEB1 (Fig. 3G, all p < 0.05).