One other study performed in HIV-negative patients [14] showed that AFP≥ 400 ng/mL is a reliable tool in the prognosis of HCC patients: HCC patients with a AFP concentration ≥400 ng/mL tend to have greater tumour size, massive or diffuse types, portal vein thrombosis, and a lower median survival rate. The gene discussed is AFP; the disease is hepatocellular carcinoma.