Moreover, the expression of AC004812.2 was positively correlated with the two m6A modification readers, IGF2BP1 and YTHDF1. Overexpression of AC004812.2 increased the mRNA levels of IGF2BP1 and YTHDF1. Thus, it is reasonable to speculate that AC004812.2 regulates osteosarcoma cell proliferation in a m6A-dependent manner. The gene discussed is YTHDF1; the disease is osteosarcoma.