Overexpression of AC004812.2 inhibited cell proliferation and increased the expression levels of IGF2BP1 and YTHDF1. In all, our findings provided a promising prognostic indicator of osteosarcoma and suggested that AC004812.2 might be an important regulator of m6A modification in osteosarcoma. The gene discussed is YTHDF1; the disease is osteosarcoma.