Phosphorylated aggregates derived from ALS brains co-transfected with WT TDP-43 into HEK293T cell-lines or neuroblastoma cell-lines show an increase in phosphorylated TDP-43 in comparison with controls, indicating that pathological TDP-43 aggregates are able to seed further aggregation. This evidence concerns the gene TARDBP and amyotrophic lateral sclerosis.