Here, we aimed to evaluate the expression of lncRNA RGMB-AS1 in NPC and explore the new mechanisms of lncRNA RGMB-AS1/FOXA1 axis on biological function and EMT in NPC, to determine whether lncRNA RGMB-AS1 can be used as a gene therapy target for NPC in vitro and in vivo. The gene discussed is RGMB; the disease is nasopharyngeal carcinoma.