TNF and intervertebral disk degenerative disorder: Here, we confirmed that the protein expression of Caspase1, NLRP3, and GSDMD-N and the LDH, TNF-α, IL-1β, and IL-6 levels were upregulated in TBHP-treated NPCs, implying that pyroptosis occurs in IDD, suggesting that degeneration of the intervertebral disc is accompanied by inflammatory death or pyroptosis of NPCs.