A large number of studies have shown that mutations in the RB gene are the cause of RB [5,6], and that predictive testing before RB is important for controlling the disease progression and subsequent treatment [7], The detection of effective biomarkers involved in tumorigenesis is essential for understanding the molecular mechanism of RB development [8]; however, research on RB pathogenesis is still limited. The gene discussed is RB1; the disease is retinoblastoma.