Moreover, TSPAN6 overexpression reduced EGF-dependent invasion using transwell migration assays (Fig. 3c); in vitro scratch assays further confirmed that TSPAN6 overexpression in these K-RAS activating mutant pancreatic cancer cells leads to impaired epithelial sheet migration (Supplementary Fig. 2f). This evidence concerns the gene TSPAN6 and pancreatic neoplasm.