Meanwhile, overexpression or knockdown of DICER1-AS1 could increase or decrease DICER1 expression of PC cells in both mRNA and protein level (Fig. 3F, Additional file 3: Figure S5C-D), which was further validated with RNA-FISH and immunofluorescence assays (Fig. 3G, Additional file 3: Figure S5E). The gene discussed is DICER1; the disease is pachyonychia congenita.