We further determined the expression of FOXQ1 in 4 human CRC cell lines (HCT116, DLD-1, HT29 and LoVo) and the normal human colonic epithelial cell line NCM460, and the results showed that compared with NCM460 cell line, the mRNA and protein expression levels of FOXQ1 in those 4 CRC cell lines were up-regulated, of which HCT116 cell line exhibited the highest expression of FOXQ1 while HT29 cell line exhibited the lowest expression of FOXQ1 (Fig. 2D, E). This evidence concerns the gene FOXQ1 and colorectal carcinoma.