Hence, in this study we focused on the underlying mechanism by which radioresistant CRC cells survive and preserve their aggressive phenotype and found that FOXQ1 may enhance the stemness and radio-resistance of CRC stem cells and ameliorate the aberrant intestinal microbiota through the FOXQ1/SIRT1/β-catenin regulatory axis. The gene discussed is FOXQ1; the disease is colorectal carcinoma.