Results of the real‐time quantitative polymerase chain reaction (RT‐qPCR) analysis showed that YAP downstream targets, including Amphiregulin (AREG) and Cyr61, were substantially upregulated in HFD tumor cells, but the mRNA level of YAP showed no significant difference between HFD and LFD cells (Figure 3B). This evidence concerns the gene CCN1 and neoplasm.