In conjunction, our data suggest that the effects of the vital medicinal herbs for alopecia treatment may be mediated mainly through the regulation of various target genes/proteins including AchE, PLA2 subtypes, NTE5, FR, NNMT, QPRT, and DAB2, and target pathways including glycerophospholipid metabolism, choline metabolism, endocytosis, nicotinate/nicotinamide metabolism, ether lipid metabolism, RAS signaling pathway, glutamatergic synapse, purine metabolism, and pyrimidine metabolism involved in the hair follicle cycle. Here, ACHE is linked to alopecia.