To simulate HIV-1 infection in cell culture, we transfected HEK293 cells or electroporated Jurkat cells, an immortalized line of human CD4+ T-cell lymphocytes, with a lentiviral vector (pfNL43-dE-EGFP) encoding the HIV-1 genome whose expression is driven by long terminal repeats. This evidence concerns the gene CD4 and HIV-1 infection.