The KEGG pathway analysis showed that the target genes of the miRNAs were primarily enriched in the miRNA in cancer, phosphatidylinositol 3-kinase (PI3K)-protein kinase B (AKT), mitogen-activated protein kinase (MAPK), Ras signaling pathway, etc. (Additional file 1: Fig. S4C). The gene discussed is AKT1; the disease is cancer.