To further evaluate the critical roles of NFKBIA in mediating the effects of NFIL3 on the activation of NF-κB and the progression of TNBC, we simultaneously overexpressed both NFKBIA and NFIL3 in Hs578T (Fig. 7A) and BT549 cells (Supplementary Fig. 7A) and detected the impact of both NFKBIA and NFIL3 overexpression on the activity of NF-κB and the proliferation, migration and invasion of both breast cancer cell lines. This evidence concerns the gene NFKB1 and breast cancer.