The loss of invasive capacity observed upon PHF8 knockdown in SKMEL-147 melanoma cells was consistently reproduced using the CRISPR-Cas9 system and two efficient small guide RNAs (sgRNAs) targeting PHF8 (sgPHF8 #1 and sgPHF8 #3) in four melanoma cell lines (SKMEL-147, 501Mel, A375, and 451Lu) (Fig. 2, A to D). The gene discussed is PHF8; the disease is melanoma.