AKT1 and hydrops fetalis: Our results showed that the protein levels of p-PI3K, p-AKT, and p-mTOR in KYSE150 and TE14 cells were significantly reduced by HF treatment at 10, 20, and 40 μM, whereas total expression levels of PI3K, AKT, and mTOR were not significantly changed (Figures 5A–C).