CTLA4 and hepatocellular carcinoma: Accordingly, we established this model to investigate the antitumor effect of CIAA on DEN\CCl4\ethanol-induced HCC mice by detecting the expression levels of AFP, CD4+, CD8+, IL-10, PD-1, CTLA-4, and AKT to explore the possible underlying molecular mechanisms of the therapeutic effect of CIAA.