CD4 and colitis: In this model, transferred CD4+CD25−CD45Rbhi effector T cells undergo both homeostatic and intestinal microbiome driven expansion that over time causes the development of severe colitis.27 To determine the effect of IL-36 signalling specifically on T cell driven colitis, we FACs sorted both wt and Il36r−/− CD4+CD25−CD45Rbhi T effector cells, transferred them to Rag1−/− recipients, and compared the pathogenesis of disease between the groups.