To overcome this obstacle, and, more importantly, enable evaluation of tumor immunity in an intact immunocompetent syngeneic system with normal immune elements, a CT26 murine microsatellite stable (MSS) colon cancer was established with EGFR5, a receptor to which both murine and human anti-EGFR can functionally interact (Fig. 1A). Here, EGFR is linked to malignant colon neoplasm.