Kaplan–Meier survival analyses showed that the elderly, normal high value of urea nitrogen, normal high value of TGF-β, normal high value of ADMA, and diabetes (we defined age ≥ 60 years as the elderly, taking the higher quartile of other measurement data as their normal high values) were significantly associated with chronic kidney disease onset in our cohort (Fig. 2). This evidence concerns the gene TGFB1 and diabetes mellitus.