CD4 and cancer: Notably, the general immune landscape, as reflected by the number of tumor-infiltrated immune cells, including CD4+ T cells, CD8+ T cells, macrophages, myeloid-derived suppressor cells (MDSCs), and dendritic cells (DCs), was mostly unaffected in Ehd2−/− mice (Supplementary Fig. 17b, d, f), suggesting that germline deletion of Ehd2 mainly affects cancer cells but does not have an apparent impact on the immune microenvironment of HCC.