TP53 and hepatocellular carcinoma: To ensure that the effect is not limited to tumors derived from MHCC97L cells, we induced HCC by hydrodynamic tail-vein injection (HDTVi) of genome-editing systems, including the CRISPR–Cas9 and Sleeping Beauty transposon systems, which enabled the deletion of Tp53 and OE of c-Myc in the liver of immunocompetent mice and formation of Tp53KO; c-MycOE HCC tumors38.