Notably, the general immune landscape, as reflected by the number of tumor-infiltrated immune cells, including CD4+ T cells, CD8+ T cells, macrophages, myeloid-derived suppressor cells (MDSCs), and dendritic cells (DCs), was mostly unaffected in Ehd2−/− mice (Supplementary Fig. 17b, d, f), suggesting that germline deletion of Ehd2 mainly affects cancer cells but does not have an apparent impact on the immune microenvironment of HCC. Here, EHD2 is linked to hepatocellular carcinoma.